Annual Reports on NMR Spectroscopy, Vol. 58 by G.A. Webb (Ed.)

By G.A. Webb (Ed.)

Content material: 1. Advances in Probe layout for Protein NMR. 2. Diffusion in smooth Polymer platforms as Approached by means of box Gradient NMR. three. How a ways Can the Sensitivity of NMR be elevated? four. functions of Low-Field NMR to nutrients technology. five. Residual Dipolar Couplings: Measurements and functions to Biomolecular stories

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Veglia, J. Am. Chem. , 2002, 124, 9392. 52. K. A. H. Wildman, D. K. Lee and A. Ramamoorthy, Biochemistry, 2003, 42, 6545. 53. T. B. Acton, K. C. Gunsalus, R. Xiao, L. C. Ma, J. Aramini, M. C. Baran, Y. W. Chiang, T. Climent, B. Cooper, N. G. Denissova, S. M. Douglas, J. K. Everett, C. K. Ho, D. Macapagal, P. K. Rajan, R. Shastry, L. Y. Shih, G. V. Swapna, M. Wilson, M. Wu, M. Gerstein, M. Inouye, J. F. Hunt and G. T. Montelione, Meth. , 2005, 394, 210. 54. H. D. W. Hill, Probes for high resolution, in: Encyclopedia of NMR, D.

1994, 66, 3849. 134. R. A. Kautz, M. E. Lacey, A. M. Wolters, F. Foret, A. G. Webb, B. L. Karger and J. V. Sweedler, J. Am. Chem. , 2001, 123, 3159. 135. M. E. Lacey, A. G. Webb and J. V. Sweedler, Anal. , 2002, 74, 4583. 136. M. E. Lacey, Z. J. Tan, A. G. Webb and J. V. Sweedler, J. Chromatogr. A, 2001, 922, 139. 137. M. E. Lacey, A. G. Webb and J. V. Sweedler, Anal. , 2000, 72, 4991. 138. Y. Li, M. E. Lacey, J. V. Sweedler and A. G. Webb, J. Magn. , 2003, 162, 133. 139. D. L. Olson, M. E. Lacey, A.

The largest S/N advantages occur at low magnetic fields or for small coils, both cases in which coil losses tend to dominate. ADVANCES IN PROBE DESIGN FOR PROTEIN NMR 25 Fig. 17. Sensitivity-enhanced 1H/15N HSQC spectra acquired in 10 min on a 50 mM sample of the catalytic domain of stromelysin using (A) a dual (15N/1H) inverse Cryoprobe (Bruker) equipped with a lock and a z-gradient and (B) a conventional triple-resonance (13C/15N/1H) inverse probe (Nalorac). 4 was realized with the Cryoprobe as compared to the conventional TXI probe.

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